Supplementation of Urea as the Nitrate Source of MS Medium and High-frequency Regeneration of Plantlets of Musa paradisiaca cv. Malnad Rasbale

Background: A consistent practice for mass multiplication of banana cv. Malnad Rasbale has been accomplished, which is an endemic cultivar Western Ghats Karnataka, India. Methods: Decrease in microbial contagion have managed by dealing with outward sterilization agents ethanol, sodium hypochlorite, mercuric chloride solution, antibiotic solutions containing cefotaxime and gentamicin. MS media augmented 350 mg/l urea as the nitrogen source substituting KNO3 NH4NO3 commonly used terroristic activities. Organogenic was supplemented a range 2-6 BAP 0.3-2.7 TDZ. Result: The addition gave eminent grades overall initiation, shoot development also rooting phase. Supplementation (4 mg/l), TDZ (1.8 mg/l) showed enthusiastic progress elongation specific concentrations.Plants established under vitro circumstances were reassigned to greenhouse after progress, they promoted field conditions.